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KMID : 1094720000050010057
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 1 p.57 ~ p.57
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Screening of microorganisms producing esterase for the production of (R)-β-Acetylmercaptoisobutyric acid from methyl (R,S)-β-acetylmercaptoisobutyrate
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Gokul Boyapati
Lee Je-Hyuk
Song Ki-Bang
Panda T.
Rhee Sang-Ki
Kim Chul-Ho
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Abstract
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(R)-β-acetylmercaptoisobutyric acid (RAM), a chiral compound, is an important intermediate for the chemical synthesis of various antihypertensive and congestive heart failure drugs. Microorganisms capable of converting (R,S)-β-acetylmercaptoisobutyric acid ((R,S)-ester) to RAM were screened from soil microorganisms. A strain ofPseudomonas sp. 1001 screened from a soil sample was selected to be the best. Cells showed an activity of 540 U/mL from culture broth and the enzyme was thermostable up to 70°C. This strain could produce RAM asymmetrically from (R,S)-ester.
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KEYWORD
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(R)-β-acetylmercaptoisobutyric acid, Pseudomonas sp, esterase, enzymatic resolution
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